PCSK9 imperceptibly affects chemokine receptor expression in vitro and in vivo

Background and Aims : Proprotein convertase subtilin/kexin type 9 (PCSK9) is a protease that gained importance in cardiovascular biology due to its regulatory action on the low-density-lipoprotein receptor (LDLR). However, PCSK9 can also act independent of LDLR cause vascular pathologies. We hypothesized affects expression chemokine-receptors CXCR4, CCR8, CCR5 CCR2 inflammatory cytokines, all major mediators inflammation, influence health.Methods: overexpressed mice evaluated effects chemokine-receptor levels different immune cells using flow-cytometry plasma cytokines multiplex analysis. Furthermore, mouse human macrophages, smooth muscle (SMCs) endothelial (ECs) were stimulated with in-vitro presence/absence an environment, investigate cytokine release ELISA.Results: It could be observed in-vivo overexpression did not have influences nor expression, although CCR8 was increased blood neutrophils, while decreased splenic T-cells. In-vitro treatment macrophages SMCs results any effect these or cytokines. Interestingly, CXCR4 ECs which coincided IL-6 release.Conclusions: Hence, we conclude are largely here investigated chemokine receptors further research required pinpoint interactions other mediators, would high fully understand mechanism by CVDs. health. Methods: ELISA. Results: release. Conclusions: